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  • Test nhanh vi khuẩn Staphylococcus

 

MASTASTAPH™

Rapid latex agglutination slide test for Staphylococcus
aureus

Staphylococcus aureus ATCC 25923: Positive in 20 seconds

Procedure:

1. Allow the MASTASTAPH Latex Reagent to
equilibrate to room temperature before use.
2. Shake well and then add 1 drop of
MASTASTAPH Latex Reagent into a circle on
the MASTASTAPH Test Card.
3. Using a clean mixing stick, pick up 2-4 average
size colonies from a fresh overnight culture plate
and emulsify the colonies in the latex, mixing
thoroughly and spreading over half the area of
the circle. Discard the stick into disinfectant.
4. Rotate and rock the card slowly and read within
1 minute.
5. Record result and dispose of the test card
safely.

Interpretation of results:
A positive result is indicated by visible aggregation
of the latex particles with a clear background. This
will occur within seconds of mixing.

A negative reaction is indicated by a milky
appearance without any visible aggregation of the
latex particles.
Faint traces of granularity or string-like appearance
without clearing of the background may be detected
in negative patterns depending on the visual acuity
of the operator.
For the specific detection of Methicillin Resistant
Staphylococcus aureus (MRSA), the MASTALEX
MRSA test kit (RST501) is recommended.

Limitations of use
Only freshly grown overnight cultures of organisms
should be used for testing directly from the plate. If
there is insufficient growth the organism should be
sub-cultured onto Mast Blood Agar Base (DM100)
or Mast Nutrient Agar (DM179) and incubated
overnight at 37°C. Organisms grown on high salt
medium such as Mannitol Salt Agar may show
signs of stringiness when mixed with the latex
reagent. Sub culturing onto Mast Blood Agar Base
(DM100) or Mast Nutrient Agar (DM179) will avoid
this problem.
Some species of staphylococci other than S. aureus
(notably S. intermedius and S. hyicus) may give
positive results in conventional coagulase test and
may also agglutinate latex reagents. Rare species
such as S. lugdunensis and S. schleiferi have been
reported as clumping factor positive. Novobiocin
resistant strains may also give false positive results
using latex based tests. Several species such as
Escherichia coli and Candida albicans are capable
of non-specifically agglutinating latex particles.
Organisms that possess immunoglobulin binding
factors may also agglutinate the sensitised latex.
Quality control
Check for signs of deterioration. Quality control
must be performed with at least one organism to
demonstrate expected performance. Do not use
the product if the result with the control organism is
incorrect. The list below recommends the control
strains which the end user can easily obtain.
Test Organisms Result
Staphylococcus aureus
ATCC 25923
Positive in 20
seconds
Normal saline solution Negative
References


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